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Preparative purification of five bioactive components from Agrimonia pilosa L edeb by high‐speed counter‐current chromatography
Author(s) -
Wang Yan,
Liu Mozhen,
Zheng Lingli,
Yin Lianhong,
Xu Lina,
Qi Yan,
Ma Xiaochi,
Liu Kexin,
Peng Jinyong
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200011
Subject(s) - chemistry , chromatography , countercurrent chromatography , high performance liquid chromatography , mass spectrometry , glycoside , organic chemistry
High‐speed counter‐current chromatography ( HSCCC ) coupled with ultraviolet ( UV ) detection or evaporative light‐scattering detection was successfully applied for preparative separation of five bioactive compounds from Agrimonia pilosa L edeb. In preliminary process, D101 macroporous resin was used to separate the crude extract of the plant and four fractions (20, 40, 50, and 60% aqueous ethanol elutions) were produced. Then, these fractions were directly subjected to HSCCC purification. Five chemicals including taxifolin‐3‐glucoside (6.4 mg), quercetin‐3‐rhamnoside (13.0 mg), tiliroside (14.7 mg), agrimonolide (21.4 mg), and tormentic acid (29.8 mg) with the purities of 94.24, 95.37, 97.42, 95.29, and 96.34% were separated from each 200 mg prepared fraction. The purities were analyzed by high‐performance liquid chromatography, and the chemical structures of the products were identified by UV detection, mass spectrometry, nuclear magnetic resonance, and the standards. This paper used a simple method to separate five bioactive compounds from A. pilosa L edeb, and it could provide a new idea for the purification of bioactive compounds from other medicinal plants.