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A novel strategy for target profiling analysis of bioactive phenylethanoid glycosides in P lantago medicinal plants using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry
Author(s) -
Qi Meng,
Xiong Aizhen,
Geng Fang,
Yang Li,
Wang Zhengtao
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200010
Subject(s) - phenylethanoid , chemistry , chromatography , tandem mass spectrometry , glycoside , quadrupole time of flight , mass spectrometry , chemometrics , stereochemistry
Phenylethanoid glycosides are a group of phenolic compounds with diverse biological activities such as hypotensive, diuretic, and hypoglycemic effects. In this study, a target profiling analysis approach using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry ( MS ) was established on the basis of parent ion scanning for m / z 161, the characteristic product ion for phenylethanoid glycosides. It was successfully employed to discriminate the chemical composition of phenylethanoid glycosides between P lantaginis H erba and P lantaginis S emen, two medicinal parts of P lantago plants, which are widely used as herbal medicine in C hina. Totally, 34 phenylethanoid glycosides were characterized and tentatively identified by their retention times, MS , and tandem quadrupole MS ( MS / MS ) data. Combined with chemometrics analysis of principal component analysis and orthogonal projection to latent structural discriminate analysis, eight of them, especially acteoside and plantamajoside, were picked out and contributed to the chemical distinction between P lantaginis H erba and P lantaginis S emen, which might be responsible for the differences in diuretic and hypotensive effects between the two medicinal parts. This new approach for target profiling provides not only a novel idea for specific analysis of active chemical constituents in the same type, but also a promising and reference method for quality evaluation of traditional C hinese medicines.