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Influence of temperature on peak shape and solvent compatibility: Implications for two‐dimensional liquid chromatography
Author(s) -
Haun Jakob,
Teutenberg Thorsten,
Schmidt Torsten C.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201101092
Subject(s) - solvent , high performance liquid chromatography , compatibility (geochemistry) , chromatography , chemistry , viscosity , analytical chemistry (journal) , materials science , organic chemistry , composite material
Solvent compatibility is a limiting factor for the success of two‐dimensional liquid chromatography (2‐D LC). In the second dimension, solvent effects can result in overpressures as well as in peak broadening or even distortion. A peak shape study was performed on a one‐dimensional high‐performance liquid chromatography (HPLC) system to simulate the impact of peak distorting solvent effects on a reversed‐phase second dimension separation operated at high temperatures. This study includes changes in injection volume, solute concentration, column inner diameter, eluent composition and oven temperature. Special attention was given to the influence of high temperatures on the solvent effects. High‐temperature HPLC (HT‐HPLC) is known to enhance second dimension separations in terms of speed, selectivity and solvent compatibility. The ability to minimise the viscosity contrast between the mobile phases of both dimensions makes HT‐HPLC a promising tool to avoid viscosity mismatch effects like (pre‐)viscous fingering. In case of our study, viscosity mismatch effects could not be observed. However, our results clearly show that the enhancement in solvent compatibility provided by the application of high temperatures does not include the elimination of solvent strength effects. The additional peak broadening and distortion caused by this effect is a potential error source for data processing in 2‐D LC.