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Titanium‐containing magnetic mesoporous silica spheres: Effective enrichment of peptides and simultaneous separation of nonphosphopeptides and phosphopeptides
Author(s) -
Li XiaoShui,
Su Xin,
Zhu Gangtian,
Zhao Yong,
Yuan BiFeng,
Guo Lin,
Feng YuQi
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201101067
Subject(s) - mesoporous material , elution , chemistry , titanium , mesoporous silica , chromatography , selectivity , magnetic nanoparticles , nanoparticle , materials science , nanotechnology , biochemistry , organic chemistry , catalysis
Protein phosphorylation is a common posttranslational modification, and involved in many cellular processes. Like endogenous peptides, endogenous phosphopeptides contain many biomarkers of preclinical screening and disease diagnosis. In this work, titanium‐containing magnetic mesoporous silica spheres were synthesized and applied for effective enrichment of peptides from both tryptic digests of standard proteins and human serum. Besides, the enriched peptides can be further separated into nonphosphopeptides and phosphopeptides by a simple elution. First, titanium‐containing magnetic mesoporous silica spheres were synthesized by a sol–gel method and found to have high surface area, narrow pore size distribution, and useful magnetic responsivity. Then, as the prepared material was used for selective capturing of phosphopeptides, it demonstrated to have higher selectivity than commercial titanium dioxide. Moreover, via combination of size‐exclusion mechanism, hydrophobic interaction, and affinity chromatography, titanium‐containing magnetic mesoporous silica spheres were successfully applied to simultaneously extract and separate nonphosphopeptides and phosphopeptides from standard protein digestion and human serum.