Premium
Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors
Author(s) -
Casoni Dorina,
Sârbu Costel
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201101032
Subject(s) - lipophilicity , chemistry , partition coefficient , protonation , methanol , extrapolation , chromatography , analytical chemistry (journal) , stereochemistry , organic chemistry , ion , mathematics , mathematical analysis
The retention behavior for a series of biogenic amines and related sympathomimetic drugs has been investigated in reversed‐phase thin‐layer chromatography using RP ‐2, RP ‐8, RP ‐18 W , and D iol stationary phase and mixtures of phosphate buffer (p H = 7.10) and methanol in different proportions as mobile phases. Several methodologies like arithmetic mean of experimental retention values, extrapolation to zero methanol concentration procedure and principal component analysis were applied to retention data values ( R M ) in order to determine relevant parameters (mean of R M − m R M , R M0 , and scores corresponding to the first principal component − PC 1/ R M respectively) encoding information on the lipophilic behavior of compounds. High similarities in lipophilicity behavior of investigated amines were highlighted by m R M and PC 1/ R M lipophilicity indices for all of the studied stationary phases. The experimental results were compared with some computed lipophilicity parameters expressed as distribution coefficients at working pH (log D ), partition coefficients (log P N , log P I , and diff(log P N–I )) concerning both neutral and fully protonated species and difference between both species, and also with various lipophilicity values (log P ) generated by different commonly used software. Significant correlations were observed between the experimental lipophilicity indices m R M respectively PC 1/ R M and diff(log P N–I ) values in all cases.