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Isolation of bioactive components from F laveria bidentis (L.) K untze using high‐speed counter‐current chromatography and time‐controlled collection method
Author(s) -
Wei Yun,
Zhang Kai,
Yin Li,
Du Jilin,
Zhang Guoliang
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201101027
Subject(s) - chromatography , chemistry , electrospray ionization , solvent , mass spectrometry , high performance liquid chromatography , volume (thermodynamics) , organic chemistry , physics , quantum mechanics
Semipreparative high‐speed counter‐current chromatography ( HSCCC ) by time‐controlled collection method was successfully applied for isolation and purification of α‐terthienyl, 5‐(3‐buten‐1‐ynyl)‐2,2′‐bithienyl, and 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl from F laveria bidentis (L.) K untze for the first time. The two‐phase solvent system composed of n ‐hexane and acetonitrile at the volume ratio of 1:1 (v/v) was used for the semipreparative HSCCC . The 5.2 mg α‐terthienyl, 2.2 mg 5‐(3‐buten‐1‐ynyl)‐2,2′‐bithienyl, and 4.3 mg 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl with the purity of 99.9, 90.2, and 92.1% were produced from 265.6 mg crude extract, respectively, and 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl was first isolated from F laveria bidentis (L.) K untze. The structures of the separated compounds were identified by electrospray‐ionization mass spectrometry and proton and carbon nuclear magnetic resonance ( 1 H‐ and 13 C‐ NMR ).