Isolation of bioactive components from F laveria bidentis (L.) K untze using high‐speed counter‐current chromatography and time‐controlled collection method

Semipreparative high‐speed counter‐current chromatography ( HSCCC ) by time‐controlled collection method was successfully applied for isolation and purification of α‐terthienyl, 5‐(3‐buten‐1‐ynyl)‐2,2′‐bithienyl, and 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl from F laveria bidentis (L.) K untze for the first time. The two‐phase solvent system composed of n ‐hexane and acetonitrile at the volume ratio of 1:1 (v/v) was used for the semipreparative HSCCC . The 5.2 mg α‐terthienyl, 2.2 mg 5‐(3‐buten‐1‐ynyl)‐2,2′‐bithienyl, and 4.3 mg 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl with the purity of 99.9, 90.2, and 92.1% were produced from 265.6 mg crude extract, respectively, and 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl was first isolated from F laveria bidentis (L.) K untze. The structures of the separated compounds were identified by electrospray‐ionization mass spectrometry and proton and carbon nuclear magnetic resonance ( 1 H‐ and 13 C‐ NMR ).