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Dye attached poly(hydroxyethyl methacrylate) cryogel for albumin depletion from human serum
Author(s) -
Andac Muge,
Galaev Igor,
Denizli Adil
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201101020
Subject(s) - methacrylate , (hydroxyethyl)methacrylate , adsorption , swelling , langmuir adsorption model , polymer chemistry , 2 hydroxyethyl methacrylate , chromatography , chemistry , human serum albumin , materials science , biocompatibility , protein adsorption , polymer , copolymer , organic chemistry , composite material
Cibacron Blue F 3GA was immobilized on poly(hydroxyethyl methacrylate) cryogel and it was used for selective and efficient depletion of albumin from human serum. The poly(hydroxyethyl methacrylate) was selected as the basic component because of its inertness, mechanical strength, chemical and biological stability, and biocompatibility. Cibacron Blue F 3GA was covalently attached to the poly(hydroxyethyl methacrylate) cryogel to produce poly(hydroxyethyl methacrylate)‐Cibacron Blue F 3GA cryogel affinity column. The poly(hydroxyethyl methacrylate)‐Cibacron Blue F 3GA cryogel was characterized with respect to gelation yield, swelling degree, total volume of macropores, Fourier Transform Infrared spectroscopy, and scanning electron microscopy. It was found that the maximum amount of adsorption (343 mg/g of dry cryogel) obtained from experimental results is very close to the calculated Langmuir adsorption capacity (345 mg/g of dry cryogel). The maximum adsorption capacity for poly(hydroxyethyl methacrylate)‐Cibacron Blue F 3GA cryogel column was obtained as 950 mg/g of dry cryogel for nondiluted serum. The adsorption capacity decreased with increasing dilution ratios while the depletion ratio of albumin remained as 77% in serum sample. Finally, the poly(hydroxyethyl methacrylate)‐Cibacron Blue F 3GA cryogel was optimized for using in the fast protein liquid chromatography system for rapid removal of the high abundant proteins from the human serum.