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Trace analysis of glyphosate in water by capillary electrophoresis on a chip with high sample volume loadability
Author(s) -
Horčičiak Michal,
Masár Marián,
Bodor Róbert,
Danč Ladislav,
Bel Peter
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100942
Subject(s) - chromatography , chemistry , capillary electrophoresis , volume (thermodynamics) , dilution , analytical chemistry (journal) , glyphosate , physics , quantum mechanics , biology , agronomy , thermodynamics
A new method for the determination of trace glyphosate (GLYP), non‐selective pesticide, by CZE with online ITP pre‐treatment of drinking waters on a column‐coupling (CC) chip has been developed. CC chip was equipped with two injection channels of 0.9 and 9.9 μmL volumes, two separation channels of 9.3 |mL total volume and a pair of conductivity detectors. A very effective ITP sample clean‐up performed in the first channel at low pH (3.2) was introduced for quick CZE resolution and detection of GLYP carried out at higher pH (6.1) in the second channel on the CC chip. The LOD for GLYP was estimated at 2.5 μg/L (15 nmol/L) using a 9.9 |mL volume of the injection channel. ITP‐CZE analyses of model and real samples have provided very favorable intra‐day (0.1‐1.2% RSD) and inter‐day (2.9% RSD) repeatabilities of the migration time for GLYP while 0.2‐6.9% RSD values were typical for the peak area data. Recoveries of GLYP in spiked drinking water varied in the range of 99‐109%. A minimum pre‐treatment of drinking water (degassing and dilution) and a short analysis time (ca. 10 min) were distinctive features of ITP‐CZE determinations of GLYP on the CC chip with high sample volume loaded, as well.