Premium
Preparation of agarose/chitosan composite supermacroporous monolithic cryogels for affinity purification of glycoproteins
Author(s) -
Sun Sijuan,
Tang Yuhai,
Fu Qiang,
Liu Xuan,
Du Wei,
Guo Li'an,
Zhao Yanding
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100940
Subject(s) - ovotransferrin , glutaraldehyde , agarose , adsorption , chitosan , egg white , chemistry , composite number , ovalbumin , genipin , chemical engineering , chromatography , matrix (chemical analysis) , aqueous solution , nuclear chemistry , materials science , composite material , biochemistry , organic chemistry , immunology , biology , immune system , engineering
Supermacroporous agarose/chitosan composite monolithic ( AC CM ) cryogels were prepared for affinity purification of the major egg white glycoproteins, ovalbumin ( OVA ), and ovotransferrin ( OVT ). The supermacroporous AC CM cryogels were produced by cryocopolymerization of agarose/chitosan blend solutions using glutaraldehyde as the cross‐linker. The 3‐aminophenlyboronic acid ligand was immobilized by covalent binding to epoxy‐group‐coupled supermacroporous AC CM cryogels. The microstructure morphologies of these cryogels were analyzed by scanning electron microscopy. The supermacroporous AC CM cryogels contained a continuous interpenetrating polymer network matrix with interconnected pores of 10–100 μm in size. The composite cryogels offered high mechanical stability and had specific recognition for glycoproteins. The maximum binding capacity of OVA adsorption from aqueous solutions was 55.6 mg/g. The matrix could be reused 11 times without significant loss in OVA adsorption capacity. The recovery yields of OVA and OVT from egg white were estimated to be 89 and 93%, respectively.