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Effective separation of dopamine from bananas on 2‐(3,4‐dimethoxyphenyl)ethylamine imprinted polymer
Author(s) -
Luliński Piotr,
Maciejewska Dorota
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100910
Subject(s) - ethylamine , nip , chemistry , molecularly imprinted polymer , chromatography , elution , polymer , polymerization , adsorption , chemisorption , solid phase extraction , nuclear chemistry , aqueous solution , methanol , extraction (chemistry) , selectivity , materials science , organic chemistry , catalysis , composite material
A 2‐(3,4‐dimethoxyphenyl)ethylamine imprinted polymer ( MIP pt ) was prepared via the precipitation polymerization together with a nonimprinted polymer (NIP). The morphology of particles was investigated by scanning electron microscopy and the specific surface areas were estimated by methylene blue adsorption (60.5 ± 3.5 and 36.9 ± 1.2 m 2 /g for MIP pt and NIP , respectively). The binding experiments were performed to determine the binding capacity of MIP pt / NIP particles toward dopamine. Next, the effects of solvents on loading, washing, and eluting steps were examined on solid‐phase extraction ( SPE ). Methanol–water 85:15 v/v (loading step), methanol (washing step), and 0.04 M aqueous ammonium acetate–methanol 30:70 v/v (eluting step) were selected as the most effective systems. Described SPE protocol was successfully applied for separation of dopamine on 2‐(3,4‐dimethoxyphenyl)ethylamine imprinted particles. Finally, the molecularly imprinted polymer was used for determination of dopamine in spiked banana extract. The total recovery of dopamine from MIP pt was equal to 88.5 ± 4.6%, but from NIP was only 12.8 ± 2.3%. The developed material and method were demonstrated to be applicable for the separation of dopamine from bananas. The commercial sorbent C 18 was not suitable to such application.