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Liquid chromatography tandem mass spectrometry for the simultaneous determination of mequindox and its metabolites in porcine tissues
Author(s) -
Zeng Dongping,
Shen Xiangguang,
He Limin,
Ding Huanzhong,
Tang Youzhi,
Sun Yongxue,
Fang Binghu,
Zeng Zhenling
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100902
Subject(s) - chromatography , chemistry , analyte , mass spectrometry , tandem mass spectrometry , detection limit , electrospray ionization , extraction (chemistry) , solid phase extraction , liquid chromatography–mass spectrometry
A rapid liquid chromatography tandem mass spectrometric method was developed for the simultaneous determination of mequindox and its five metabolites (2‐isoethanol mequindox, 2‐isoethanol 1‐desoxymequindox, 1‐desoxymequindox, 1,4‐bisdesoxymequindox, and 2‐isoethanol bisdesoxymequindox) in porcine muscle, liver, and kidney, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. The method involved acid hydrolysis, purification by solid‐phase extraction, and subsequent analysis with liquid chromatography tandem mass spectrometry using electrospray ionization operated in positive polarity with a total run time of 15 min. The decision limit values of five analytes in porcine tissues ranged from 0.6 to 2.9 μg/kg, and the detection capability values ranged from 1.2 to 5.7 μg/kg. The results of the inter‐day study, which was performed by fortifying porcine muscle (2, 4, and 8 μg/kg), liver, and kidney (10, 20, and 40 μg/kg) samples on three separate days, showed that the accuracy of the method for the various analytes ranged between 75.3 and 107.2% with relative standard deviation less than 12% for each analyte.