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Determination of hyperoside and isoquercitrin in rat plasma by membrane‐protected micro‐solid‐phase extraction with high‐performance liquid chromatography
Author(s) -
Yin XiaoYing,
Luo YongMing,
Fu JianJiang,
Zhong YouQuan,
Liu QingShan
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100867
Subject(s) - hyperoside , chromatography , extraction (chemistry) , solid phase extraction , membrane , high performance liquid chromatography , chemistry , quercitrin , sorbent , adsorption , rutin , organic chemistry , biochemistry , antioxidant
A novel method, micro‐solid‐phase extraction based on membrane‐protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro‐solid‐phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120 μg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22 μg/mL, respectively. Compared with traditional methods, solid‐phase extraction, liquid–liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples.