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Determination of trace indium in urine after preconcentration with a chelating‐resin‐packed minicolumn
Author(s) -
Liu HuiMing,
Chang ChingYao,
Wu ChiaChan,
Wei JianMing,
Chen WeiYu,
Yeh ChengTsung
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100866
Subject(s) - chemistry , chromatography , elution , detection limit , analyte , column chromatography , graphite furnace atomic absorption , chelating resin , ion exchange resin , certified reference materials , analytical chemistry (journal) , ion , metal ions in aqueous solution , inorganic chemistry , organic chemistry
A simple and rapid chelating‐resin‐packed column has been developed for preconcentration of trace indium in biological samples. A large‐sized urine sample was pumped through a minicolumn at a flow rate of 1.0 mL/min by using a peristaltic pump, and the eluents were analyzed using graphite furnace atomic absorption spectrometry ( GFAAS ). Four commercially available chelating resins including C helex‐100, A mberlite IRC ‐50, D uolite GT ‐73, and C elite 545‐ AW were studied for evaluating the indium sorption performance. Several parameters, such as pH, resin amount, eluent volume, eluent flow rate, and the volume of sample, were investigated and optimized. A 100–200 mL of the sample was loaded into a column containing 1.2 g of wet C helex‐100 and subjected to the ion‐exchange procedure. The retained analytes were eluted with 5.0 mL of 0.1 M HNO 3 and quantified by GFAAS . The correlation coefficient in the range 10–250 ng/mL was of 0.9994. The limit of detection of the proposed method was 2.75 ng/mL. The method developed was successfully applied to analysis of spiked urine samples with good recoveries of 93–103% ( n = 6) and reproducibility (relative standard deviation < 4.9%). The accuracy of procedure was confirmed by indium determination in spiked certified reference materials.