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Ultra‐high pressure LC determination of glucosamine in shrimp by‐products and migration tests of chitosan films
Author(s) -
SanchesSilva Ana,
Ribeiro Tiago,
Albuquerque Tânia G.,
Paseiro Perfecto,
Sendón Raquel,
de Quirós Ana Bernaldo,
LópezCervantes Jaime,
SánchezMachado Dalia I.,
Valdez Herlinda Soto,
Angulo Inmaculada,
Aurrekoetxea Goizane P.,
Costa Helena S.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100855
Subject(s) - chitosan , derivatization , chromatography , chemistry , shrimp , glucosamine , chloroformate , hydrolysis , repeatability , detection limit , ultrapure water , high performance liquid chromatography , organic chemistry , materials science , nanotechnology , fishery , biology
Chitosan, a multiple applications molecule, was isolated from shrimp by‐products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9‐fluorenylmethyl chloroformate (Fmoc‐Cl). Ultra‐high‐pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).