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Fast profiling of chemical constituents in Yiqing Capsule by ultra‐performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry
Author(s) -
Zheng GuoDong,
Li Kai,
Li Yueshan,
Liu EHu
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100736
Subject(s) - chemistry , chromatography , formic acid , electrospray ionization , tandem mass spectrometry , radix (gastropod) , mass spectrometry , sanguinarine , high performance liquid chromatography , anthraquinones , alkaloid , organic chemistry , botany , biology
An ultra‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC‐ESI‐MS n ) has been developed for structural characterization and identification of multi‐constituents in Yiqing Capsule, a well‐known combined herbal remedy prepared from the extract mixtures of Rhizoma Coptidis, Radix et Rhizoma Rhei and Radix Scutellariae. The UPLC analysis was performed on an Agilent ZorBax SB‐C 18 column (4.6 mm×50 mm, 1.8 μm) and gradient elution of 0.1% formic acid solution and acetonitrile in 16 min. Based on their retention times and mass spectra in comparison with the data from standards or references, a total of 29 compounds including 3 phenolic acids and 4 anthraquinones from Radix et Rhizoma Rhei, 8 alkaloids from Rhizoma Coptidis and 14 flavonoids from Radix Scutellariae were unambiguously identified or tentatively characterized in the complex system. The MS data and fragmentation information of two isomers of feruloylquinic acid were first reported in Radix et Rhizoma Rhei and in Yiqing Capsules. This study is expected to be accepted as an effective and reliable pattern for comprehensive and systematic characterization of this commonly used Chinese herbal preparation.