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pH‐based cation exchange chromatography in the capture and elution of monoclonal antibodies
Author(s) -
Ng Paul K.,
Snyder Mark A.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100720
Subject(s) - chromatography , chemistry , elution , monoclonal antibody , ion chromatography , isoelectric point , high performance liquid chromatography , adsorption , affinity chromatography , salt (chemistry) , displacement chromatography , isoelectric focusing , column chromatography , ion exchange , hydrophilic interaction chromatography , antibody , biochemistry , organic chemistry , ion , immunology , biology , enzyme
Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH‐based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia™ S), which has high binding capacity. A three‐column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50‐cycle study.