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Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting
Author(s) -
Camenzuli Michelle,
Ritchie Harald J.,
Ladine James R.,
Shalliker R. Andrew
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100687
Subject(s) - elution , chromatography , flow (mathematics) , inlet , chemistry , column (typography) , volumetric flow rate , sample (material) , analytical chemistry (journal) , materials science , mechanics , computer science , geology , telecommunications , physics , frame (networking) , geomorphology
Active flow management in the form of curtain flow sample introduction and segmented outlet flow control has been shown to enable sample to elute through a chromatography column under the principles of the “infinite diameter column”. Such an elution process avoids the detrimental effects of the heterogeneity of particle‐packed chromatographic columns by injecting the sample directly into the radial core region of the column, thus avoiding wall effects. The process described herein illustrates how the principles of the infinite diameter column can be applied using conventional injection devices suitable for long‐term analysis that requires robust protocols. Using this approach, sensitivity in separation was 2.5 times greater than conventional chromatography, yielding a product at twice the concentration. Benefits of curtain flow injection are thus relevant to both preparative‐scale and analytical‐scale separations.