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Tentative identification of new metabolites of epimedin C by liquid chromatography–mass spectrometry
Author(s) -
Liu Minyan,
Zhao Shaohua,
Wang Zongquan,
Wang Hongtao,
Shi Xiaowei,
Lü Ziming,
Xu Honghui,
Wang Hairong,
Du Yingfeng,
Zhang Lantong
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100581
Subject(s) - chemistry , metabolite , hydroxylation , mass spectrometry , chromatography , demethylation , electrospray , biotransformation , glucuronic acid , metabolic pathway , metabolism , biochemistry , enzyme , gene expression , gene , polysaccharide , dna methylation
Epimedin C is one of the major bioactive constituents of Herba Epimedii . The aim of this study is to characterize and elucidate the structure of metabolites in the rat after administration of epimedin C. Metabolite identification was performed using a predictive multiple reaction monitoring–information dependent acquisition–enhanced product ion (pMRM‐IDA‐EPI) scan in positive ion mode on a hybrid triple quadrupole‐linear ion trap mass spectrometer. A total of 18 metabolites were characterized by the changes in their protonated molecular masses, their MS/MS spectrum and their retention times compared with those of the parent drug. The results reveal possible metabolite profiles of epimedin C in rats; the metabolic pathways including hydrolysis, hydroxylation, dehydrogenation, demethylation and conjugation with glucuronic acid and different sugars were observed. This study provides a practical approach for rapidly identifying complicated metabolites, a methodology that could be widely applied for the structural characterization of metabolites of other compounds.