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Molecularly imprinted polymer for selective extraction of 3‐methylflavone‐8‐carboxylic acid from human urine followed by its determination using zwitterionic hydrophilic interaction liquid chromatography
Author(s) -
Rao Ramisetti N.,
Maurya Pawan K.,
Kuntamukkala Ramakrishna,
Vitthal Wani D.,
Talluri Murali V. N. K.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100560
Subject(s) - chemistry , molecularly imprinted polymer , chromatography , extraction (chemistry) , metabolite , adsorption , solid phase extraction , carboxylic acid , detection limit , matrix (chemical analysis) , sample preparation , selectivity , urine , organic chemistry , biochemistry , catalysis
A novel water‐compatible molecularly imprinted SPE combined with zwitterionic hydrophilic interaction liquid chromatography method for selective extraction and determination of 3‐methylflavone‐8‐carboxylic acid (MFA), the main active metabolite of flavoxate in human urine, was developed and validated. The effects of progenic solvents, pH, cross linker and amount of monomer were studied to optimize the efficiency and selectivity. The molecularly imprinted polymer showed good specific adsorption capacity with an optimum of 200 μmol/g at pH 7.5 and selective extraction of MFA from human urine. The recovery of MFA from human urine was >98%. The lower limit of quantification was 1.20 μg/mL. The proposed method overcomes the matrix effects of endogenous substances generally encountered during direct analysis of urine sample.