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Electromembrane extraction of peptides – Fundamental studies on the supported liquid membrane
Author(s) -
Seip Knut Fredrik,
Stigsson Jeanette,
Gjelstad Astrid,
Balchen Marte,
PedersenBjergaard Stig
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100558
Subject(s) - chromatography , extraction (chemistry) , chemistry , membrane , reproducibility , dilution , high performance liquid chromatography , aqueous solution , liquid–liquid extraction , selectivity , sample preparation , partition coefficient , analytical chemistry (journal) , organic chemistry , biochemistry , physics , thermodynamics , catalysis
A large screening of different components in the supported liquid membrane (SLM) in electromembrane extraction (EME) was performed to test the extraction efficiency on eight model peptides. Electromembrane extraction from a 500 μL acidified aqueous sample containing the model peptides in the concentration 10 μg/mL was used. Extraction time was 5 min with an electric potential of 10 V and 900 rpm agitation of the sample vial. The samples were extracted through a hollow fiber‐based SLM with different compositions of organic solvents and carriers. A small volume of acidified acceptor solution (25 μL) was after extraction analyzed directly, or with some dilution, on CE or HPLC. This article has identified mono‐ or di‐substituted phosphate groups as the prominent group of carrier molecules needed to obtain acceptable recoveries. For the organic solvents, primary alcohols and ketones have shown promise regarding recovery and reproducibility, with some differences in selectivity. A new composition of the SLM, namely 2‐octanone and tridecyl phosphate (90:10 w/w) has proved to give higher extraction recoveries and lower standard deviation than SLMs previously reported in the literature.