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Preparative isolation and purification of senkyunolide‐I, senkyunolide‐H and ferulic acid from Rhizoma Chuanxiong using counter‐current chromatography
Author(s) -
Wei Yun,
Hu Jia,
Li Hao,
Liu Jiangang
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100547
Subject(s) - chemistry , chromatography , countercurrent chromatography , ferulic acid , ethyl acetate , partition coefficient , elution , hexane , solvent , high performance liquid chromatography , methanol , organic chemistry
Three active compounds, senkyunolide‐I, senkyunolide‐H and ferulic acid (FA), were successfully isolated and purified from the extracts of Rhizoma Chuanxiong by counter‐current chromatography (CCC). Based on the principle of the partition coefficient values ( k ) for target compounds and the separation factor (α) between target compounds, the two‐phase solvent system that contains n ‐hexane–ethyl acetate–methanol–water at an optimized volume ratio of 3:7:4:6 v/v was selected for the CCC separation, and the lower phase was employed as the mobile phase in the head‐to‐tail elution mode. In a single run, 400 mg of the crude extract yielded pure senkyunolide‐I (6.4 mg), senkyunolide‐H (1.7 mg) and FA (4.4 mg) with the purities of 98, 93 and 99%, respectively. The CCC fractions were analyzed by high‐performance liquid chromatography, and the structures of the three active compounds were identified by MS and 1 H NMR.