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Silica‐coated La 0.75 Sr 0.25 MnO 3 nanoparticles for magnetically driven DNA isolation
Author(s) -
Trachtová Štěpánka,
Kaman Ondřej,
Španová Alena,
Veverka Pavel,
Pollert Emil,
Rittich Bohuslav
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100442
Subject(s) - nanoparticle , dna , adsorption , colloid , magnetic nanoparticles , aqueous solution , chemistry , chromatography , nanotechnology , materials science , nuclear chemistry , analytical chemistry (journal) , chemical engineering , biochemistry , organic chemistry , engineering
Magnetic La 0.75 Sr 0.25 MnO 3 nanoparticles possessing an approximately 20‐nm‐thick silica shell (LSMO(0.25)@SiO 2 ) were characterised and tested for the isolation of PCR‐ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16% PEG 6000–2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.