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LC‐MS/MS profiling for detection of endogenous steroids and prostaglandins in tissue samples
Author(s) -
Surowiec Izabella,
Koc Mariusz,
Antti Henrik,
Wikström Pernilla,
Moritz Thomas
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100436
Subject(s) - chemistry , chromatography , steroid , electrospray , hormone , solid phase extraction , detection limit , ion suppression in liquid chromatography–mass spectrometry , mass spectrometry , matrix (chemical analysis) , steroid hormone , endogeny , sample preparation , liquid chromatography–mass spectrometry , biochemistry
Roles of steroid hormones, and compounds that can influence their levels in cells, are of increasing interest in e.g. cancer research, partly because resistance to hormone therapies often complicates treatment. To elucidate the processes involved, the hormones and related compounds need to be accurately measured. Reversed‐phase liquid chromatography with dynamic multiple reaction monitoring mass spectrometric detection in electrospray mode is capable of providing such measurements. Therefore, LC‐MS/MS was developed for sensitive, selective analysis of 11 steroid hormones, cholesterol and two prostaglandins. The effects of the tissue matrix, and solid‐phase extraction (SPE) sample clean‐up, on the LC‐MS/MS signals of the hormones were also investigated. The results show that the developed LC‐MS/MS method, following SPE clean‐up to reduce matrix interference, can detect selected steroids in extracts of mouse tissues. The method provides linear measurements of the steroids at concentrations up to few ng/μL, and limits of detection in the range 0.03–0.2 pg/μL (for some compounds lower than those of previously reported methods).