Premium
An integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection
Author(s) -
Nakajima Hizuru,
Okuma Yukiko,
Morioka Kazuhiro,
Miyake Mayo,
Hemmi Akihide,
Tobita Tatsuya,
Yahiro Masayuki,
Yokoyama Daisuke,
Adachi Chihaya,
Soh Nobuaki,
Nakano Koji,
Xue Shuhua,
Zeng Hulie,
Uchiyama Katsumi,
Imato Toshihiko
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100429
Subject(s) - oled , detection limit , fluorescence , optoelectronics , microfluidics , materials science , photolithography , luminescence , analytical chemistry (journal) , diode , chemistry , optics , chromatography , nanotechnology , physics , layer (electronics)
A fluorescence detection system for a microfluidic device using an organic light‐emitting diode (OLED) as the excitation light source and a charge‐coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm 2 was 13 μW (1.7 mW/cm 2 ). The fluorescence detection system consisted of the OLED device, two band‐pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow‐based enzyme‐linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit ( S / N =3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96‐well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.