Premium
Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography
Author(s) -
Zhang Bo,
Sheng Qianying,
Li Xiuling,
Liang Qi,
Yan Jingyu,
Liang Xinmiao
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100427
Subject(s) - glycopeptide , chromatography , chemistry , mass spectrometry , fractionation , glycan , glycosylation , proteomics , glycoprotein , biochemistry , gene , antibiotics
Comprehensive glycoprotein characterization based on mass spectrometry (MS) is challenging because of low concentration of glycopeptides and suppression effect of abundant non‐glycosylated peptides in MS. Therefore, it is vital to enrich glycopeptides before MS analysis. A new method was developed to selectively enrich glycopeptides from complex sample by coupling C18 fractionation with titanium dioxide (TiO 2 ) enrichment. The new method allows to selectively enrich N‐linked glycopeptides with various glycan forms and different sequence lengths. Compared with single TiO 2 method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. Further application of this method to mixture of non‐glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples.