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Determination of bisphenol A with effective pretreatment medium using automated column‐switching HPLC with fluorescence detection
Author(s) -
Tanigawa Tetsuya,
Watabe Yoshiyuki,
Kubo Takuya,
Hosoya Ken
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100418
Subject(s) - chromatography , high performance liquid chromatography , detection limit , repeatability , chemistry , bisphenol a , column (typography) , computer science , telecommunications , organic chemistry , frame (networking) , epoxy
A practical way for reducing contaminants, such as humic acids, and solving column‐clogging problem in environmental water analysis with liquid chromatography is proposed. Detection interference by contamination is one of the most important issues of the environmental analyses. Moreover, due to the recent smaller diameter and fine particle size of an analytical column for HPLC system, a column‐clogging problem is another practical difficulty as well. We found it possible to solve these problems by employing column‐switching HPLC, which consists of a pretreatment column containing surface‐modified polymer particles and flow changeover valves for cleaning the remaining matrices in the pretreatment column prior to analysis. This method was successfully applied to actual HPLC‐fluorescence detection of bisphenol A. Limit of detection (LOD) in real sample was <0.7 ng/L. Repeatability was around 1.4% and recovery was around 97% or more. A particular pressure increase was not observed in 150 repeated analyses of real river water samples.