Premium
Size separation and size determination of liposomes
Author(s) -
SakaiKato Kumiko,
Ota Shigenori,
Hyodo Kenji,
Ishihara Hiroshi,
Kikuchi Hiroshi,
Kawanishi Toru
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100417
Subject(s) - liposome , capillary action , chromatography , analytical chemistry (journal) , light scattering , materials science , chemistry , scattering , optics , nanotechnology , composite material , physics
We developed a method for separating liposomes by size and determining their average diameters. Liposomes with different average diameters were separated on a monolithic silica capillary column, and the size of the liposomes corresponding to each peak was determined online with a dynamic light scattering detector coupled to the capillary liquid chromatography system. The calculated diameters for the separated liposomes were similar to the diameter values measured in batch mode. We demonstrate that this combination of a monolithic capillary column and light scattering detection could be used for size separation of liposomes and could provide more details about average diameters than batch‐mode analysis.