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Comparison of capillary electrophoretic techniques for analysis and characterization of metallothioneins
Author(s) -
Zalewska Marta,
Bizoń Anna,
Milnerowicz Halina
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100379
Subject(s) - metallothionein , capillary electrophoresis , isoelectric focusing , chromatography , chemistry , gene isoform , proteomics , mass spectrometry , capillary action , electrophoresis , capillary electrophoresis–mass spectrometry , resolution (logic) , biochemistry , enzyme , materials science , electrospray ionization , artificial intelligence , computer science , composite material , gene
To explore and understand the significance of individual metallothionein isoforms, the methods of their identification are needed. Separation of these isoforms requires a high resolution technique which can exploit very small differences in mass, charge, and hydrophobicity. In this report, three different techniques of CE were analyzed and used for metallothionein separation: detection using capillary gel electrophoresis, capillary zone electrophoresis, and capillary isoelectric focusing. Also, three different metallothionein samples were used from horse kidney, rabbit liver, and human liver. We identified metallothionein isoforms based on the determination of their relative molecular masses, on the charge differences in different pH buffers, and based on the p I value. Methods used in this report allow metallothionein identification, permit to quantify the purity and content of its isoforms, and allow studying its polymerization. This report supports and endorses the increased application of CE methodology in proteomics.