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Chemical fingerprint analysis of Fritillaria delavayi Franch. by high‐performance liquid chromatography
Author(s) -
Duan Baozhong,
Huang Linfang,
Chen Shilin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100373
Subject(s) - chemistry , chromatography , high performance liquid chromatography , hypoxanthine , decoction , inosine , fingerprint (computing) , uridine , adenosine , botany , biochemistry , biology , enzyme , gene , rna , computer security , computer science
Bulbus of Fritillaria delavayi Franch. is the most commonly used antitussive and apophlegmatic in China and commonly prepared by water decoction. In this study, a novel and reliable method of high‐performance liquid chromatography (HPLC) was developed both for quantitative analysis of 10 bioactive compounds (uracil, cytidine, inosine, uridine, guanosine, thymidine, adenosine, hypoxanthine, adenine and 2‐deoxyadenosine) and chemical fingerprint analysis of F. delavayi Franch. In quantitative analysis, 10 compounds showed good regressions ( R 2 > 0.9982) within test ranges and the recovery of the method was in the range of 96.33–104.51%. In the fingerprint analysis, 11 characteristic peaks were selected to evaluate the similarities of F. delavayi Franch. samples, and the HPLC chromatograms of 16 samples from different regions of China showed similar patterns. The results from the experiment demonstrated that the combinations of the quantitative and chromatographic fingerprint analysis offer an efficient way to evaluate the quality consistency of F. delavayi Franch.