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Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry
Author(s) -
Chen Qinhua,
Li Na,
Zhang Wenpeng,
Chen Jing,
Chen Zilin
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100359
Subject(s) - vindoline , catharanthus roseus , chromatography , chemistry , capillary electrophoresis , mass spectrometry , ammonium acetate , detection limit , acetic acid , high performance liquid chromatography , vinblastine , biochemistry , medicine , surgery , chemotherapy
Catharanthus roseus is an important dicotyledonous medicinal plant that contains various anticancer components, such as vinblastine (VLB) and its monomeric precursors (vindoline and catharanthine). A capillary electrophoresis–mass spectrometry (CE‐MS) approach for the simultaneous determination of three components was developed in this work. Baseline separation for three components was achieved by using a running buffer consisting of 20 mM ammonium acetate and 1.5% acetic acid in <20 min. Quantification of three components was assigned in positive‐ion mode at a protonated molecular ion [M+H] + . The CE‐MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the above components. The detection limits of VLB, catharanthine and vindoline are 0.8, 0.1 and 0.1 μg/mL, respectively. The precision was not more than 4.54% and the mean recovery of the analytes was 95.04–97.04%. The CE‐MS method was successfully applied to determine VLB and its monomeric precursors in real sample C. roseus .