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Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures
Author(s) -
Sarazin Cédric,
Thiébaut Didier,
Sassiat Patrick,
Vial Jérôme
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100332
Subject(s) - supercritical fluid chromatography , chromatography , supercritical fluid , chemistry , supercritical carbon dioxide , carbon dioxide , phase (matter) , diffusion , packed bed , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , thermodynamics , physics
The implementation of columns packed with sub‐2 μm particles in supercritical fluid chromatography (SFC) is described using neat carbon dioxide as the mobile phase. A conventional supercritical fluid chromatograph was slightly modified to reduce extra column band broadening. Performances of a column packed with 1.8 μm C18‐bonded silica particles in SFC using neat carbon dioxide as the mobile phase were compared with results obtained in ultra high performance liquid chromatography (UHPLC) using a dedicated chromatograph. As expected and usual in SFC, higher linear velocities than in UHPLC must be applied in order to reach optimal efficiency owing to higher diffusion coefficient of solutes in the mobile phase; similar numbers of theoretical plates were obtained with both techniques. Very fast separations of hydrocarbons are presented using two different alkyl‐bonded silica columns.