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Preparative separation of minor saponins from Platycodi Radix by high‐speed counter‐current chromatography
Author(s) -
Ha In J.,
Kang Minseok,
Na Yun C.,
Park Youmie,
Kim Yeong S.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100326
Subject(s) - countercurrent chromatography , chemistry , chromatography , saponin , rhamnose , fraction (chemistry) , high performance liquid chromatography , glycosidic bond , arabinose , xylose , organic chemistry , polysaccharide , medicine , alternative medicine , pathology , fermentation , enzyme
Platycosides (PSs), the saponins found in the root of Platycodon grandiflorum (Jacq.) A. DC. (Platycodi Radix), are typically composed of oleanene backbones with two side chains; one is a 3‐ O ‐glucose linked by a glycosidic bond, and the other is a 28‐ O ‐arabinose‐rhamnose‐xylose‐apiose linked by an ester bond. Minor saponins, acetylated isomers of the major saponin on either the 2″ or 3″ position of rhamnose, were isolated from Platycodi Radix using a multi‐step process including high‐speed counter‐current chromatography (HSCCC) and preparative reversed‐phase high‐performance liquid chromatography (RP‐HPLC). After the separation of the major components, the enriched minor saponin fraction was used for this study. A two‐phase solvent system consisting of chloroform–methanol–isopropanol–water (3:2:2:3, v/v) was used for HSCCC. HSCCC separation of the enriched minor saponin fraction yielded 2″‐ O ‐acetylplatycodin D, 3″‐ O ‐acetylpolygalacin D, 2″‐ O ‐acetylpolygalacin and a mixture of 3″‐ O ‐acetylplatycodin D and polygalacin D. The mixture fraction from HSCCC separation was further purified by preparative RP‐HPLC, giving 3″‐ O ‐acetylplatycodin D and polygalacin D at a purity of over 98.9%. The developed method provides the preparative and rapid separation of minor saponins in the crude extract of Platycodi Radix. To the best of our knowledge, this is the first on the separation of acetylated PSs by HSCCC.