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A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins
Author(s) -
Haider Syed R.,
Sharp Barry L.,
Reid Helen J.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100315
Subject(s) - tris , tricine , chromatography , glycine , chemistry , electrophoresis , barbital , biochemistry , amino acid
This paper compares different buffer systems for the electrophoretic separation of the five most abundant serum proteins on native‐PAGE gel and cellulose membranes. A modified Tris‐tricine system was shown to be superior for the separation of these serum proteins in a 7% m/v native‐PAGE gel as compared with the traditionally used Tris‐glycine and Tris‐tricine methods. This modified Tris‐tricine buffer system was also employed for the separation of serum proteins using a cellulose acetate membrane and very effective separation was observed as compared with the traditionally used Tris‐barbital and Tris‐glycine buffer systems.