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Rapid simultaneous determination of isoflavones in Radix puerariae using high‐performance liquid chromatography–triple quadrupole mass spectrometry with novel shell‐type column
Author(s) -
Du Gang,
Zhao Haiyu,
Song Yuelin,
Zhang Qingwen,
Wang Yitao
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100295
Subject(s) - chromatography , chemistry , radix (gastropod) , mass spectrometry , isoflavones , analyte , high performance liquid chromatography , triple quadrupole mass spectrometer , calibration curve , elution , selected reaction monitoring , analytical chemistry (journal) , detection limit , tandem mass spectrometry , biochemistry , botany , biology
A high‐performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS) method was developed for rapid determination of 13 isoflavones in Radix puerariae . A novel shell‐type column, namely Kinetex core‐shell C 18 column (50 mm×2.1 mm id, 2.6 μm), and gradient elution were used during the analysis. The chromatographic peaks of 13 investigated compounds were identified by comparing their retention time and MS data with the related reference compounds. Multiple‐reaction monitoring (MRM) was employed for the quantitative analysis with negative ionization mode. All calibration curves showed good linearity ( r 2 >0.9990) within test ranges. The LOD and LOQ were lower than 0.017 and 0.873 μg/mL on column, respectively. The intra‐ and inter‐day precisions for 13 analytes were <1.17 and 2.17%, respectively, and the recoveries were 93.1–104.4%. The validated method was applied for quantitative analysis of 13 isoflavones in 7 species of Radix puerariae . The result demonstrated that HPLC‐MS/MS system with Kinetex column could be a promising analytical tool for the determination of isoflavones in traditional Chinese medicines, which is helpful for comprehensive evaluation of quality of R. puerariae .