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Silica‐based and organic monolithic capillary columns for LC: Recent trends in proteomics
Author(s) -
Rozenbrand Johan,
van Bennekom Wouter P.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100294
Subject(s) - proteomics , chromatography , capillary action , polystyrene , monolithic hplc column , column (typography) , methacrylate , chemistry , materials science , nanotechnology , high performance liquid chromatography , computer science , polymer , organic chemistry , telecommunications , biochemistry , frame (networking) , copolymer , composite material , gene
The use of monolithic liquid chromatography (LC) columns for proteomics, covering the scientific literature from 2004 to the beginning of 2011, is reviewed. Attention is paid to recent developments in column technology and materials, focusing on silica‐based and organic (polystyrene and methacrylate) monolithic capillary columns for proteomics. The applicability of these columns is illustrated by examples of the analysis of (complex) protein digests and proteins conveniently summarized in tables. Furthermore, characteristics of column materials are compared and future trends and prospects are presented.