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Separation and sweeping of metal ions with EDTA in CZE‐ESI‐MS
Author(s) -
Quirino Joselito P.,
Haddad Paul R.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100290
Subject(s) - chemistry , ethylenediaminetetraacetic acid , capillary electrophoresis , metal ions in aqueous solution , metal , chelation , electrospray ionization , chromatography , analytical chemistry (journal) , manganese , mass spectrometry , elution , zinc , ion , copper , electrophoresis , electrolyte , electrospray , magnesium , inorganic chemistry , organic chemistry , electrode
Ethylenediaminetetraacetic acid (EDTA) in the background electrolyte (BGE) of capillary zone electrophoresis coupled to an electrospray ionization mass spectrometer is presented as an approach for the determination of metal ions. Significant signals for the metal–ligand complexes were observed even when EDTA was continually eluted from the capillary during the entire electrophoretic run. The signal‐to‐noise ratio was improved by the addition of ammonia to the sheath liquid and by using an acquisition m / z range above the m / z of EDTA. The LODs for the test metal ions (i.e. calcium(II), manganese(II), and zinc(II)) with conventional injection were around 1–2 mg/L with corrected peak areas that are linear from 8 to 100 mg/L. The presence of EDTA in the BGE was critical not only for the separation but also for sweeping via complexation as an on‐line sample concentration technique. The peak height of the test metal ions was improved at least tenfold with sweeping via EDTA complexation and yielded LODs in the μg/L range.