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Capacitively coupled contactless conductivity detection as an alternative detection mode in CE for the analysis of kanamycin sulphate and its related substances
Author(s) -
ElAttug Mohamed N.,
Adams Erwin,
Hoogmartens Jos,
Van Schepdael Ann
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100267
Subject(s) - chemistry , chromatography , detection limit , kanamycin , ammonium acetate , conductivity , ammonium bromide , bromide , analytical chemistry (journal) , high performance liquid chromatography , inorganic chemistry , pulmonary surfactant , antibiotics , biochemistry
A method was developed to determine simultaneously kanamycin, its related substances and sulphate in kanamycin sulphate using capacitively coupled contactless conductivity detection. Kanamycin is an aminoglycoside antibiotic that lacks a strong UV‐absorbing chromophore. Due to its physicochemical properties, CE in combination with capacitively coupled contactless conductivity detection was chosen. The separation method uses a BGE composed of 40 mM 2‐( N ‐morpholino)ethanesulphonic acid monohydrate and 40 mM L ‐histidine, pH 6.35. A 0.6 mM N ‐cetyltrimethyl ammonium bromide (CTAB) solution was added as electroosmotic flow modifier in a concentration below the critical micellar concentration (CMC). Ammonium acetate 50 mg/L was used as internal standard. In total, 30 kV was applied in reverse polarity on a fused‐silica capillary (65/41 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity ( R 2 =0.9999) for kanamycin. It shows a good precision expressed as RSD on the relative peak areas equal to 0.3 and 1.1% for intra‐day and inter‐day precision, respectively. The LOD and LOQ are 0.7 and 2.3 mg/L, respectively. Similarly, for sulphate, a good linearity ( R 2 =0.9996) and precision (RSD 0.4 and 0.6% for intra‐day and inter‐day, respectively) were obtained.