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Iron oxide nanoparticle coating of organic polymer‐based monolithic columns for phosphopeptide enrichment
Author(s) -
Krenkova Jana,
Foret Frantisek
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100256
Subject(s) - phosphopeptide , nanoparticle , iron oxide nanoparticles , polymer , coating , iron oxide , oxide , chemistry , organic polymer , materials science , chemical engineering , chromatography , nanotechnology , organic chemistry , peptide , biochemistry , engineering
A new monolithic capillary column with an iron oxide nanoparticle coating has been developed for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were prepared by a co‐precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent electrostatic interactions of citrate ions on the surface of iron oxide nanoparticles with a quaternary amine functionalized poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monolith. A high dynamic binding capacity of 86 μmol/mL column volume was measured with an adenosine‐5′‐triphosphate. Performance of the monolithic column was demonstrated with the efficient and selective enrichment of phosphopeptides from peptide mixtures of α‐casein and β‐casein digests and their MALDI/MS characterization in off‐line mode.