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Brush‐like copolymer as a physically adsorbed coating for protein separation by capillary electrophoresis
Author(s) -
Zhou Dan,
Tan Lin,
Xiang Lina,
Zeng Rongju,
Cao Fuhu,
Zhu Xiaoxi,
Wang Yanmei
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100255
Subject(s) - copolymer , coating , capillary electrophoresis , protein adsorption , capillary action , chemistry , ethylene glycol , chromatography , adsorption , chemical engineering , methacrylate , protein purification , materials science , polymer chemistry , polymer , organic chemistry , composite material , engineering
A brush‐like copolymer consisting of poly(ethylene glycol) methyl ether methacrylate and N , N ‐dimethylacrylamide (PEGMA‐DMA) was synthesized and used as a novel static physically adsorbed coating for protein separation by capillary electrophoresis for the first time, in order to stabilize electroosmotic flow (EOF) and suppress adsorption of proteins onto the capillary wall. Very stable and low EOF was obtained in PEGMA‐DMA‐coated capillary at pH 2.2–7.8. The effects of molar ratio of PEGMA to DMA, copolymer molecular mass, and pH on the separation of basic proteins were discussed. A comparative study of bare capillary with PEGMA‐DMA‐coated capillary for protein separation was also performed. The basic proteins could be well separated in PEGMA‐DMA‐coated capillary over the investigated pH range of 2.8–6.8 with good repeatability and high separation efficiency because the copolymer coating combines good protein‐resistant property of PEG side chains with excellent coating ability of PDMA‐contained backbone. Finally, the coating was successfully applied to the fast separation of other protein samples, such as protein mixture and egg white, which reveals that it is a potential coating for further proteomics analysis.