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Characterization and identification of multiple constituents in Yinhuang granules by high‐performance liquid chromatography with diode‐array and time‐of‐flight mass spectrometry detection
Author(s) -
Liu EHu,
Liu Qun,
Chu Chu,
Li Ping
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100254
Subject(s) - chromatography , mass spectrometry , characterization (materials science) , high performance liquid chromatography , time of flight mass spectrometry , chemistry , chromatography detector , analytical chemistry (journal) , materials science , nanotechnology , organic chemistry , ion , ionization
A fast high‐performance liquid chromatography (HPLC) method with diode‐array detection (DAD) and time‐of‐flight mass spectrometry (TOF/MS) has been developed for the analysis of multi‐constituent in Yinhuang granules, a well‐known combined herbal remedy prepared from the extract mixtures of Flos Lonicerae and Radix Scutellariae. The fast HPLC analysis was performed on an Agilent ZorBax SB‐C 18 column (4.6×50 mm, 1.8 μm) and 0.2% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution in 17 min, which is five times faster than the performance of conventional columns packed with 5.0 μm particles. With various fragmentor voltages in TOF/MS, accurate mass measurements (<5 ppm error) for molecular ions and characteristic fragment ions represented reliable identification criteria for different constituents. A total of 28 compounds, including nine phenolic acids, three iridoid glycosides and nine saponins from Flos Lonicerae and seven flavonoids from Radix Scutellariae, were identified or tentatively characterized in the extract of Yinhuang granules. The established fast HPLC‐DAD‐TOF/MS method turns out to be useful and efficient for quality control of this commonly used Chinese herbal preparation.