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Fluorescence derivatization of single‐walled carbon nanotubes for analysis by means of conventional CE‐LIF
Author(s) -
Yamamoto Tatsuhiro,
Murayama Shuhei,
Kato Masaru
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100247
Subject(s) - derivatization , carbon nanotube , fluorescence , reagent , selectivity , materials science , triphenylene , laser induced fluorescence , dispersant , chemistry , nanotechnology , combinatorial chemistry , chromatography , mass spectrometry , organic chemistry , dispersion (optics) , molecule , physics , quantum mechanics , optics , catalysis
Single‐walled carbon nanotubes (SWNTs) are now widely used in many fields, and while many analytical methods for SWNTs have been reported, there are few practical analytical methods that combine the necessary levels of selectivity and sensitivity. We have developed a highly sensitive separation method for fluorescence‐derivatized SWNTs by means of conventional CE with laser‐induced fluorescence. First, SWNTs were dispersed using a triphenylene derivative into the water, and the excess dispersant was removed by nitric acid treatment. The dispersed SWNTs were then derivatized with a fluorescence reagent, 4‐aminofluorescein. Finally, the derivatized SWNTs were analyzed using a conventional apparatus CE‐LIF detection. The SWNTs migrated within 20 min. The detection sensitivity of SWNTs was improved by about 170 times with LIF detection as compared with UV detection. We anticipate that the derivatized SWNTs can also be detected with high sensitivity using LC.