Premium
Synthesis and application of clindamycin succinate as a novel chiral selector for capillary electrophoresis
Author(s) -
Wu Jianfeng,
Liu Peng,
Wang Li,
Tian Hailin,
Wang Qingwei,
Zhang Shengyong
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100228
Subject(s) - capillary electrophoresis , chemistry , chromatography , resolution (logic) , ofloxacin , enantiomer , analyte , tris , clindamycin , selectivity , electrophoresis , capillary action , chiral resolution , stereochemistry , organic chemistry , materials science , antibiotics , biochemistry , artificial intelligence , composite material , ciprofloxacin , computer science , catalysis
A novel chiral selector, clindamycin succinate, was synthesized and first used as a chiral selector in capillary electrophoresis (CE). The chiral resolution ability of this kind of clindamycin derivation was studied by CE using some racemic drugs as model analytes. From the experimental results, it was found that both resolution and selectivity of the selector were dependent on the following parameters: concentration of chiral selectors, pH of the running buffer, temperature of the capillary column, applied voltage and organic modifier used. The results show that the chiral selector possesses high resolution toward some racemic drugs, including ofloxacin, chlorphenamine, tryptophan, propranolol, sotalol and metoprolol. Excellent chiral resolution of these tested drugs was achieved under the optimal conditions of 50 mM clindamycin succinate, 10% MeOH v/v, 50 mM Tris buffer, pH 4.0, at 22 kV and 20°C within 25 min.