Premium
One‐step isolation of γ‐oryzanol from rice bran oil by non‐aqueous hydrostatic countercurrent chromatography
Author(s) -
Angelis Apostolis,
Urbain Aurélie,
Halabalaki Maria,
Aligiannis Nektarios,
Skaltsounis AlexiosLeandros
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100192
Subject(s) - chromatography , chemistry , rice bran oil , countercurrent chromatography , high performance liquid chromatography , fractionation , aqueous solution , fraction (chemistry) , bran , raw material , organic chemistry
The value‐added γ‐oryzanol was purified in one step from crude rice bran oil (RBO) using a preparative hydrostatic countercurrent chromatography (hydrostatic CCC) method, operating in the dual mode. The fractionation was performed using a non‐aqueous biphasic solvent system consisting of heptane–acetonitrile–butanol (1.8:1.4:0.7, v/v/v), leading rapidly to the target compounds. Transfer of the analytical CCC method to large‐scale isolation was also carried out yielding a high quantity‐high purity fraction of γ‐oryzanol. In addition, a fraction of hydroxylated triterpene alcohol ferulates (polar γ‐oryzanol) was clearly separated and obtained. Furthermore, a fast HPLC‐APCI(±)‐HRMS method was developed and applied for the identification of γ‐oryzanol as well as the polar γ‐oryzanol in RBO and the resulting fractions. The purity of γ‐oryzanol fraction was estimated as 97% based on HPLC‐APCI‐HRMS analysis.