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Separation of adrenergic amines in Citrus aurantium L. var. amara by capillary electrochromatography using a novel monolithic stationary phase
Author(s) -
Chizzali Evelyn,
Nischang Ivo,
Ganzera Markus
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100186
Subject(s) - chemistry , chromatography , capillary electrochromatography , ammonium acetate , micellar electrokinetic chromatography , repeatability , electrochromatography , alkaloid , rutaecarpine , tyramine , phenethylamine , capillary electrophoresis , high performance liquid chromatography , organic chemistry , stereochemistry , biochemistry
This manuscript reports on the use of capillary electrochromatography for the determination of tyramine, (±) synephrine, and (±) octopamine, the major alkaloids in bitter orange peel. A novel methacrylate‐based monolithic stationary phase was prepared by UV‐photopolymerization in 100 μm id fused‐silica capillaries. It facilitated the quantitative assessment of alkaloids with a mobile phase comprising aqueous 10 mM ammonium acetate in ACN and isopropanol. Applied voltage and temperature were 25 kV and 25°C, and samples were injected in electrokinetic mode. The method reported herein revealed adequate sensitivity (LOD ≤0.6 μg/mL), repeatability (σ rel ≤4.1%), accuracy (recovery rates between 95.2 and 102.2%), and precision (intra‐day variation ≤5.7%, inter‐day variation ≤4.1%). The application of the CEC assay on C. aurantium var. amara plant material and dietary supplements, which usually are advertised for slimming properties, indicated that synephrine (0.17–0.82%) is the dominant alkaloid.