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Thin‐layer chromatographic plates with monolithic layer of silica: Production, physical–chemical characteristics, separation capabilities
Author(s) -
Frolova Anastasiya M.,
Konovalova Olga Y.,
Loginova Lidia P.,
Bulgakova Alena V.,
Boichenko Alexander P.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100178
Subject(s) - sorption , sorbent , cetylpyridinium chloride , chromatography , layer (electronics) , chemistry , kinetics , chemical engineering , silica gel , materials science , adsorption , analytical chemistry (journal) , organic chemistry , pulmonary surfactant , biochemistry , physics , quantum mechanics , engineering
The technique for production of thin‐layer chromatographic plates with fixed monolithic layer of sorbent was developed on the basis of investigation of factors affecting sorption capacity, sorption kinetics and mechanical stability of monoliths. The optimal reaction mixture for sol–gel synthesis of monoliths consisted of tetraethoxysilane, buffer solution with pH 7.4, N,N ‐dimethylformamide, ethanol, polyethyleneglycol with molecular weight 1000 and cetylpyridinium chloride in molar ratio 1.0:4.6:1.4:7.6:0.26:8×10 −3 . On the basis of analysis of sorption kinetics of malachite green on the monoliths it was concluded that mechanism of sorption includes chemisorption. The optimized conditions for fixing the monolithic layer on the carrier and its drying allow obtaining undisturbed monolithic layer, which was used for test mixtures separation. The increase of monolithic layer thickness in comparison with ultrathin‐layer chromatographic plates allows detecting visually at reasonable concentrations and loaded sample volumes the spots of food and synthetic dyes.