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Simultaneous analysis of acetaminophen, p ‐aminophenol and aspirin metabolites by hydrophilic interaction and strong anion exchange capillary liquid chromatography coupled to amperometric detection
Author(s) -
Zheng Minmin,
Wu Yimin,
Lu Lanxiang,
Ding Kang,
Tang Fengxiang,
Lin Zian,
Wu Xiaoping
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100163
Subject(s) - chromatography , chemistry , detection limit , analyte , amperometry , hydrophilic interaction chromatography , salicylic acid , acetonitrile , high performance liquid chromatography , electrode , biochemistry , electrochemistry
A simple and sensitive method has been developed for the simultaneous determination of polar nonsteroidal pharmaceuticals and metabolites, including acetaminophen, p ‐aminophenol and several aspirin metabolites (salicylic acid, gentisic acid, salicyluric acid and 2,3‐dihydroxybenzoic acid), by capillary liquid chromatography with amperometric detection. Using a capillary monolithic column with mixed mode stationary phases and a mobile phase composed of acetonitrile and Tris buffer, rapid separation of six polar analytes was achieved within 8 min, and a hydrophilic interaction and strong anion exchange separation mechanism were exhibited. Method detection limits of six analytes ranged from 10 to 50 ng/mL. In terms of precision, the intra‐ and interday relative standard deviation values in all analytes never exceeded 3.1% for migration time and 8.9% for peak areas, respectively. This method provided a simple, rapid and cost‐effective approach for the analysis of polar pharmaceuticals. The applicability of the method in pharmacokinetics was verified by spiking human serum samples with the compounds and analyzing the recoveries.