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Disposable pipette extraction for gas chromatographic determination of codeine, morphine, and 6‐monoacetylmorphine in vitreous humor
Author(s) -
Kovatsi Leda,
Rentifis Konstantinos,
Giannakis Dimitrios,
Njau Samuel,
Samanidou Victoria
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100124
Subject(s) - chromatography , pipette , codeine , forensic toxicology , sample preparation , drugs of abuse , extraction (chemistry) , biological fluids , solid phase extraction , chemistry , morphine , medicine , drug , pharmacology
The availability of a sensitive and rapid analytical method for the determination of opiates, and other substances of forensic interest, in a variety of biological specimens is of utmost importance to forensic laboratories. Solid‐phase extraction is very popular in the pre‐treatment of forensic samples. Nevertheless, a new approach, disposable pipette extraction (DPX), is gaining increasing interest in sample preparation. DPX has already been applied to the analysis of drugs of abuse in common biological matrices, such as urine and blood, but has not yet been evaluated on alternative biological samples, such as vitreous humor. The objective of this study was to evaluate the applicability of DPX on the analysis of opiates in vitreous humor. The currently developed method is fast, reliable, and easy to perform. The sensitivity, precision, and accuracy are satisfactory. Recoveries obtained are within the range of 72–91%, whereas the sample volume of vitreous humor required is only 100 μL.