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Optimisation of a solid‐phase microextraction method for the analysis of nicotine in hair
Author(s) -
Brčić Karačonji Irena,
Zimić Lukrica,
Brajenović Nataša,
Skender Ljiljana
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100109
Subject(s) - solid phase microextraction , chromatography , detection limit , chemistry , extraction (chemistry) , gas chromatography , gas chromatography–mass spectrometry , nicotine , solid phase extraction , hair analysis , analytical chemistry (journal) , mass spectrometry , neuroscience , biology , medicine , alternative medicine , pathology
A headspace solid‐phase microextraction method (HS‐SPME) followed by gas chromatography with mass spectrometric detection (GC/MS) has been developed for the determination of low concentrations of nicotine in hair. Parameters affecting the SPME procedure including type of fiber coating, extraction mode, extraction temperature and time, desorption time, stirring, and salt addition have been evaluated and optimised. The method provided good linearity ( r 2 ≥0.9980) over the concentration range tested (0.2–20 ng/mg) and low detection limit (0.02 ng/mg). Precision expressed as relative standard deviation was <10%. The average accuracy was 95%. The proposed method was used to determine hair nicotine levels in 100 children in order to assess exposure to environmental tobacco smoke (ETS). The described HS‐SPME procedure is fast, simple, sensitive, and solvent‐free and is therefore suitable for studies involving ETS exposure assessment.