Premium
QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS
Author(s) -
Flores María Isabel Alarcón,
RomeroGonzález Roberto,
Frenich Antonia Garrido,
Vidal José Luis Martínez
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100093
Subject(s) - quechers , chromatography , chemistry , electrospray ionization , tandem mass spectrometry , extraction (chemistry) , electrospray , mass spectrometry , detection limit , pesticide residue , pesticide , agronomy , biology
A new method has been developed and validated for the simultaneous analysis of different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The compounds were extracted using a QuEChERS‐based method (acronym of quick, easy, cheap, effective, rugged and safe). The separation and determination of the selected phytohormones were carried out by ultra high‐performance liquid chromatography coupled to tandem mass spectrometry (UHPLC‐MS/MS), using electrospray ionization source (ESI) in positive and negative ion modes. The method was validated and mean recoveries were evaluated at three concentration levels (50, 100 and 250 μg/kg), ranging from 75 to 110% at the three levels assayed. Intra‐ and interday precisions, expressed as relative standard deviations (RSDs), were lower than 20 and 25%, respectively. Limits of quantification (LOQs) were equal or lower than 10 μg/kg. The developed procedure was applied to seven courgette samples, and naphthylacetic acid, naphthylacetamide and benzyladenine were found in some of the analysed samples.