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Quantitation of free and total bisphenol A in human urine using liquid chromatography‐tandem mass spectrometry
Author(s) -
Fox Stephen D.,
Falk Roni T.,
Veenstra Timothy D.,
Issaq Haleem J.
Publication year - 2011
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201100087
Subject(s) - chromatography , chemistry , detection limit , dansyl chloride , bisphenol a , high performance liquid chromatography , bisphenol , glucuronide , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , urine , solid phase extraction , derivatization , epoxy , organic chemistry , biochemistry
Bisphenol A (BPA) is employed in the synthesis of polycarbonate plastics and epoxy resins and is widely used in consumer products including as a coating for the inside of almost all food and beverage containers and thermal‐imaging paper. Bisphenol A is considered to have important health implications because it possesses weak estrogenic activity and can leach from storage containers resulting in its consumption by both humans and animals. It is metabolized in the body and excreted into urine as a glucuronide derivative. In this report, we present an accurate, selective, sensitive, and reproducible high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for the quantitation of BPA in human urine, which is not prone to exogenous contamination. BPA‐glucuronide is hydrolyzed enzymatically, extracted with toluene, derivatized with dansyl chloride, and the BPA‐(dansyl) 2 derivative is analyzed using reversed‐phase HPLC/MS/MS. Calibration was linear to 50 ng/mL with a limit of quantitation of 50 pg/mL and a limit of detection of 5 pg/mL.